Biodun Olujimi, senator representing Ekiti north and Duro Faseyi, her counterpart in Ekiti south, have lost their reelection bid to their All Progressives Congress (APC) opponents.
The senators are members of the Peoples Democratic Party (PDP).
In Ekiti south, Adedayo Adeyeye, a former minister, polled 77,621 votes to defeat Olujimi who polled 53,741 votes.
Adeyeye was a former spokesman of PDP. He dumped the party after he lost the governorship ticket to Kolapo Olusola, deputy of Ayodele Fayose, former governor of Ekiti.
Olujimi whom he defeated also contested the PDP gubernatorial primary election but stepped down for the ex-PDP spokesman in the dying minute.
She emerged minority leader of the upper legislative chamber after Godswill Akpabio, a senator from Akwa Ibom defected to the APC.
In Ekiti north, Olubunmi Adetumbi of the APC, polled 60,689 votes to defeat Faseyi, who got 49,209 votes.